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Resource Summary

The ITSF provides a service for Telethon and general research community. The facility produces and purified monoclonal antibodies at GLP production level. The applied principles of liquid chromatography ensure rapid production high purity, clinical grade monoclonal antibodies, suitable for biochemical and pre-clinical studies. The product (hybrid cells or antibodies source material) produced by the ITSF, provided by individual investigators or from our portfolio is up-scale, purified and quality controlled from standard up to pre-clinical grade quality (functional grade) by a battery of biochemical analysis.

Contact information

Director ITSF:

Dr Alberto Horenstein, PhD
Address: Via Santena 19
City code: 10126
City: Turin (TO), Italy
Phone +39 011 6961734
Fax +39 011 6966155
E-mail alberto.horenstein@unito.it

 

Facilities

Location:

The facility is located at The Laboratory of Immunogenetics-Purification Unit-CeRMS (University of Turin and Molinette Hospital) - Via Santena 19 10126-TURIN-Italy.

Services

  • Generation of hybrid cells producing monoclonal antibodies
  • Characterising antibodies by EIA, RIA, indirect inmunofluorescence and confocal microscopy
  • Isotyping of the produced monoclonal antibodies
  • Scaling-up production [10 mg - 1 g level]
  • Preparative HPLC purification (standard and detoxified functional grade)
  • Size Exclusion Chromatography
  • Gel electrophoresis (SDS-PAGE; isoelectrofocusing)
  • Production of ascites and hybridoma supernatants

Fee Schedules/Rates

SERVICES Standard rate Telethon

Immunization, Fusion, subcloning

€ 3,000

50%

Production/scaling-up (ascites and culture sup)

€ 1,000

50%

Standard purification

€ 500/≤10 mg

50%

Functional grade purification

€ 800/≤10 mg

50%

Quality control (standard)

€ 400

50%

Quality control (high grade)

€ 700

50%

Additional services: LAL Test (endotoxins)

€ 300

50%

 

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